Single Radial Immunodiffusion of Undiluted Serum Proteins
نویسندگان
چکیده
منابع مشابه
Single radial immunodiffusion of undiluted serum proteins.
A simple method is described for quantitative determination of lgG, IgA, 1gM, a2M, and other proteins without dilution of the sample. Strips of gelatinized cellulose acetate spread with antiserum are used as a supporting medium. The method is accurate, highly reproducible, and seems particularly suitable for routine work because it permits determination of hypo-, normal, and hyperglobulinemia o...
متن کاملComparison of radial immunodiffusion and laser nephelometry for quantitating some serum proteins.
After quantitating immunoglobulins G, A, and M and complement C3c and C4 in serum by using a laser nephelometer coupled with a data processor, I compared these results with values obtained by an early-readout radial immunodiffusion method. Day-to-day precision was better for nephelometry than for radial immunodiffusion for all proteins analyzed. The average coefficient of variation was 6.0% for...
متن کاملLaser nephelometric measurement of seven serum proteins compared with radial immunodiffusion.
Quantification of immunoglobulins IgG, IgA, IgM, C3 complement, siderophilin, alpha 2-macroglobulin and haptoglobin in serum by a Behring laser nephelometer coupled with a date processing apparatus was compared with values obtained by the radial immunodiffusion method of Mancini. The precision was not improved by the use of the laser nephelometer as compared to Mancini radial immunodiffusion, b...
متن کاملDetermination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay.
Two immunochemical methods for determination of thyroxine-binding globulin in human serum were developed, in which the purified globulin and monospecific antiserum to it are used. One method, based on radial immunodiffusion, has good precision and values for analytical recovery. Reference values obtained for men were 9.8-17.8 mg/liter and for women 11.3-20.5 mg/liter. The sex-related difference...
متن کاملNormative values of serum immunoglobulins by single radial immunodiffusion: a review.
Many previous studies of normative values in adults have suggested that race, age, sex, and environment all have significant effects on the mean values for IgG, IgA, IgM, and IgE in various groups of individuals. Single radial immunodiffusion is the technique most widely used to quantitate immunoglobulins of the three major classes (IgG, IgM, and IgA) in sera. Measurements have been expressed i...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1972
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/18.3.237